Functional analysis of pcpme6 from oomycete plant pathogen Phytophthora capsici.
Identifieur interne : 001800 ( Main/Exploration ); précédent : 001799; suivant : 001801Functional analysis of pcpme6 from oomycete plant pathogen Phytophthora capsici.
Auteurs : Baozhen Feng [République populaire de Chine] ; Peiqian Li ; Hemei Wang ; Xiuguo ZhangSource :
- Microbial pathogenesis [ 1096-1208 ]
Descripteurs français
- KwdFr :
- Capsicum (microbiologie), Carboxylic ester hydrolases (génétique), Carboxylic ester hydrolases (métabolisme), Données de séquences moléculaires (MeSH), Feuilles de plante (microbiologie), Maladies des plantes (microbiologie), Phytophthora (enzymologie), Phytophthora (génétique), Phytophthora (pathogénicité), Protéines d'algue (génétique), Protéines d'algue (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Virulence (MeSH).
- MESH :
- enzymologie : Phytophthora.
- génétique : Carboxylic ester hydrolases, Phytophthora, Protéines d'algue.
- microbiologie : Capsicum, Feuilles de plante, Maladies des plantes.
- métabolisme : Carboxylic ester hydrolases, Protéines d'algue.
- pathogénicité : Phytophthora.
- Données de séquences moléculaires, Régulation de l'expression des gènes fongiques, Séquence d'acides aminés, Séquence nucléotidique, Virulence.
English descriptors
- KwdEn :
- Algal Proteins (genetics), Algal Proteins (metabolism), Amino Acid Sequence (MeSH), Base Sequence (MeSH), Capsicum (microbiology), Carboxylic Ester Hydrolases (genetics), Carboxylic Ester Hydrolases (metabolism), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Phytophthora (enzymology), Phytophthora (genetics), Phytophthora (pathogenicity), Plant Diseases (microbiology), Plant Leaves (microbiology), Virulence (MeSH).
- MESH :
- chemical , genetics : Algal Proteins, Carboxylic Ester Hydrolases.
- chemical , metabolism : Algal Proteins, Carboxylic Ester Hydrolases.
- enzymology : Phytophthora.
- genetics : Phytophthora.
- microbiology : Capsicum, Plant Diseases, Plant Leaves.
- pathogenicity : Phytophthora.
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Fungal, Molecular Sequence Data, Virulence.
Abstract
The Phytophthora capsici inflicts damage on numerous crop plants by secreting a series of pectinase including pectin methylesterase (PME). We identified a PME gene (pcpme6) from a genomic library of a highly virulent P. capsici strain SD33 which had an encoded a polypeptide of 348 amino acid residues with a predicted molecular mass of 38.18 kDa. We also confirmed that pcpme6 was increasingly expressed during symptom development following P. capsici infection of pepper leaves. The wild-type protein (PCPME6) ca. 50 kDa was obtained from pcpme6 expression, and PME activity trend in PCPME6-treated pepper leaves increased with symptom development. PCPME6 degraded leaf cell walls, resulting in the production of necrotic lesions. Mutation of Asp residues in active sites within pcpme6 affected PCPME6 activity and its virulence on pepper leaves. Results show that pcpme6 is a gene within the pme gene family that is important for pathogenesis of P. capsici on pepper.
DOI: 10.1016/j.micpath.2010.03.004
PubMed: 20227480
Affiliations:
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scheme="KwdEn" xml:lang="en"><term>Algal Proteins (genetics)</term><term>Algal Proteins (metabolism)</term><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Capsicum (microbiology)</term><term>Carboxylic Ester Hydrolases (genetics)</term><term>Carboxylic Ester Hydrolases (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phytophthora (enzymology)</term><term>Phytophthora (genetics)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Capsicum (microbiologie)</term><term>Carboxylic ester hydrolases (génétique)</term><term>Carboxylic ester hydrolases (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (microbiologie)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (enzymologie)</term><term>Phytophthora (génétique)</term><term>Phytophthora (pathogénicité)</term><term>Protéines d'algue (génétique)</term><term>Protéines d'algue (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Algal Proteins</term><term>Carboxylic Ester Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Algal Proteins</term><term>Carboxylic Ester Hydrolases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxylic ester hydrolases</term><term>Phytophthora</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Capsicum</term><term>Feuilles de plante</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Capsicum</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxylic ester hydrolases</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Gene Expression Regulation, Fungal</term><term>Molecular Sequence Data</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes fongiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Phytophthora capsici inflicts damage on numerous crop plants by secreting a series of pectinase including pectin methylesterase (PME). We identified a PME gene (pcpme6) from a genomic library of a highly virulent P. capsici strain SD33 which had an encoded a polypeptide of 348 amino acid residues with a predicted molecular mass of 38.18 kDa. We also confirmed that pcpme6 was increasingly expressed during symptom development following P. capsici infection of pepper leaves. The wild-type protein (PCPME6) ca. 50 kDa was obtained from pcpme6 expression, and PME activity trend in PCPME6-treated pepper leaves increased with symptom development. PCPME6 degraded leaf cell walls, resulting in the production of necrotic lesions. Mutation of Asp residues in active sites within pcpme6 affected PCPME6 activity and its virulence on pepper leaves. Results show that pcpme6 is a gene within the pme gene family that is important for pathogenesis of P. capsici on pepper.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20227480</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2010</Year><Month>05</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1208</ISSN><JournalIssue CitedMedium="Internet"><Volume>49</Volume><Issue>1-2</Issue><PubDate><MedlineDate>2010 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>Microbial pathogenesis</Title><ISOAbbreviation>Microb Pathog</ISOAbbreviation></Journal><ArticleTitle>Functional analysis of pcpme6 from oomycete plant pathogen Phytophthora capsici.</ArticleTitle><Pagination><MedlinePgn>23-31</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micpath.2010.03.004</ELocationID><Abstract><AbstractText>The Phytophthora capsici inflicts damage on numerous crop plants by secreting a series of pectinase including pectin methylesterase (PME). We identified a PME gene (pcpme6) from a genomic library of a highly virulent P. capsici strain SD33 which had an encoded a polypeptide of 348 amino acid residues with a predicted molecular mass of 38.18 kDa. We also confirmed that pcpme6 was increasingly expressed during symptom development following P. capsici infection of pepper leaves. The wild-type protein (PCPME6) ca. 50 kDa was obtained from pcpme6 expression, and PME activity trend in PCPME6-treated pepper leaves increased with symptom development. PCPME6 degraded leaf cell walls, resulting in the production of necrotic lesions. Mutation of Asp residues in active sites within pcpme6 affected PCPME6 activity and its virulence on pepper leaves. Results show that pcpme6 is a gene within the pme gene family that is important for pathogenesis of P. capsici on pepper.</AbstractText><CopyrightInformation>Copyright 2010 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Baozhen</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Shandong Agricultural University, No. 61 Daizong street, Tai'an 271018, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Peiqian</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hemei</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xiuguo</ForeName><Initials>X</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>FJ213430</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>03</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microb Pathog</MedlineTA><NlmUniqueID>8606191</NlmUniqueID><ISSNLinking>0882-4010</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.1.-</RegistryNumber><NameOfSubstance UI="D002265">Carboxylic Ester Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.1.11</RegistryNumber><NameOfSubstance UI="C022323">pectinesterase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002265" MajorTopicYN="N">Carboxylic Ester Hydrolases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence 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PubStatus="revised"><Year>2010</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>03</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>10</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20227480</ArticleId><ArticleId IdType="pii">S0882-4010(10)00041-0</ArticleId><ArticleId IdType="doi">10.1016/j.micpath.2010.03.004</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Li, Peiqian" sort="Li, Peiqian" uniqKey="Li P" first="Peiqian" last="Li">Peiqian Li</name><name sortKey="Wang, Hemei" sort="Wang, Hemei" uniqKey="Wang H" first="Hemei" last="Wang">Hemei Wang</name><name sortKey="Zhang, Xiuguo" sort="Zhang, Xiuguo" uniqKey="Zhang X" first="Xiuguo" last="Zhang">Xiuguo Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Feng, Baozhen" sort="Feng, Baozhen" uniqKey="Feng B" first="Baozhen" last="Feng">Baozhen Feng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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